QGY7703 cells were seeded into a 6‑well plate at a density of 1x105 cells/ml, QGY7703 cell apoptosis induced by elemene. Cell proliferation inhibition rate (%) was calculated as: (A in treatment group‑A in control group)/(A in control group‑A in blank control)x100. The absorbance (A) of each well was measured by ELISA Reader/Microplate Reader DR‑200Bs (Huawen Machinery & Electronics Co., Wuxi, China). A total of 150 µl DMSO was then added into each well, and the plate was oscillated on a shaker at low speed for 10 min to allow the crystals to dissolve. After the cells were cultured for 48 h, 20 µl MTT was added and incubated for 4 h, and then the supernatant was removed. The cells' apoptotic rate increased dose‑dependently with elemene concentration, and the difference was statistically significant (P200 µl. Cell proliferation was measured with MTT assay, cell apoptosis and cell cycle were analyzed by flow cytometry, and GSTP1 gene methylation was analyzed by methlation‑specific polymerase chain reaction. QGY7703 cells were treated with different elemene concentrations.
The present study aimed to investigate elemene's effects on cell proliferation, apoptosis, and the cell cycle in the hepatocellular carcinoma (HCC) cell line, QYG7703, and to investigate GSTP1 gene methylation change in QGY7703 cells after being treated with elemene to explore whether elemene reversed the abnormal GSTP1 gene methylation. China Received DecemAccepted JanuDOI: 10.3892/ol.2016.4243 Abstract. Demethylation effects of elemene on the GSTP1 gene in HCC cell line QGY7703 BAOQIANG WU, YONG JIANG, FENG ZHU, DONGLIN SUN and HONGJUN HUANG Department of Hepatobiliary Surgery, The First People's Hospital of Changzhou, Changzhou, Jiangsu 213003, P.R.
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